Pathogenesis, transmission and response to re-exposure of SARS-CoV-2 in domestic cats

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Pathogenesis, transmission and response to re-exposure of SARS-CoV-2 in domestic cats

Added 9 June 2020

Bosco-Rauth, A. M. et al. (2020)  Pathogenesis, transmission and response to re-exposure of SARS-CoV-2 in domestic cats. bioRxiv 2020.05.28.120998; https://doi.org/10.1101/2020.05.28.120998

This is another small experimental study looking at response to exposure and transmission to SARS-CoV-2 in both cats and dogs. There were three parts to this study.


ANIMALSInterventionResults
Cats – Group 1 (3 cats)Inoculated intranasally with SARS-CoV-2None of the cats became clinically ill
Nasal and oral swabs taken on days 1,3, 5, 7, 10 and 14 for virus isolation and qPCRCats shed virus for up to 5 days with peak shedding at day 3
Viral levels from nasal swabs were higher than from oral swabs
Blood collected weekly for 6 weeks for ELISA antibody measurementInfected cats all developed detectable antibody by day 7, reaching or exceeding 1:2560 by day 14. Antibody titres stayed stable or increased between days 28-42
Re-exposed to virus at day 28Moderate increase in antibody titres noted 14 days after exposure
Further swabs taken 1, 3, 7, 10 14No viral shedding detected after re-exposure
Euthanased at day 42All three cats had mild lung changes, including mild interstitial lymphocytic pneumonia with peribronchiolar and
perivascular lymphocytic cuffing and alveolar histiocytosis.
Cats – Group 2 (4 cats)Two cats inoculated with SARS-CoV-2 intranasally then co-housed with two other cats 48 hrs later.
Nasal and oral swabs taken on days 1, 3, 5, 7, 10 and 14Inoculated cats shed virus as group 1.

Contact cats started shedding within 24 hours of being housed with infected cats but had more prolonged shedding with peak at 7 days post exposure

Blood collected weekly for 6 weeks All cats developed an antibody response
2 Inoculated cats euthanased on day 5Virus was isolated from trachea, nasal turbinates and oesophagus but was not found in the lung or other organs of
either cat.
Dogs (3 dogs)Inoculated intranasally with SARS-CoV-2None of the dogs developed signs of disease
Nasal and oral swabs taken on days 1-5, 7, 10 and 14Viral shedding not detected
Blood collected weekly for 6 weeksDogs developed neutralizing antibodies by 14 DPI and peaked at 21 DPI with titres between 1:40-1:80

The authors conclude that cats are highly susceptible to subclinical infection, with a prolonged period of oral and nasal viral shedding, that is not accompanied by clinical signs. The study again shows that cats can become infected through direct contact with other infected cats. The authors also state that cats develop a robust neutralizing antibody response that prevented re-infection to a second viral challenge.

The dogs in this study did not appear to shed virus but did mount a low-level antibody response.

This is another study that has been made available on bioRxix before peer review. It should be remembered that this study reports on experimental infection in laboratory bred (and pathogen free) animals, this will not be the case with domestic cats where secondary infections may explain clinical signs.

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